Influence of Gliding Arc Cold Plasma Based on Argon, Helium and Compact Air on Bactria Around the Wound: Experimental Investigation

Background: Today, the use of cold plasma and its applications have taken into consideration. The plasma is a mixture of active oxygen and negative ions. This compound has a practical effect on the elimination of all types of bacteria and viruses. The cold plasma feature is essential and very useful in disinfecting processes. In this investigation, we plan to study the effect of gliding arc plasma on the destruction of two type of bacteria called S.aureus and P. aeruginosa which funded in the around the injury. Methods: Plasma was irradiated to culturing bacteria environment over the different period. After applying the plasma, the Petri dishes were incubated at 37 ° C for 12 hours to grow the bacteria in the incubator. On the following day, colony formation was counted, then gliding arc plasma was induced at the atmospheric pressure to the bacteria that was implanted. Plasma effects were evaluated for the destruction of bacteria. Three types of gas were used for the creation of the plasma. Results: Radiation of a high dose of plasma, caused significant bacterial destruction and decreased bacterial colonies. After about 1 minute, 99.9% of the S.aureus bacteria were destroyed. The best results are obtained in the presence of argon plasma and compressed air such that after 1 minute of plasma irradiation and compressed air, 90% of S.Aureus bacteria were destroyed at a concentration of 102 bacteria, while helium had little effect on bacteria. Conclusion: The study also shows that gram-positive bacteria (S.Aureus) are more sensitive to plasma than P. gonorrhea (P. aeruginosa) and disappear faster.